 |
【著書】
| レーニンジャー
の新生化学（第5版）「上」，廣川書店（2010）（翻訳分担） |
| 食物科学概論，的場輝佳編，朝倉書店 （2003） |
| 生化学 基礎の基礎，化学同人（2002） |
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| 酵素ハンドブック（第3版），朝倉書店, 2008 |
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【受賞歴】
- Rockefeller Foundation Fellowship (1984)
- Rockefeller Foundation Research Grant (1986) "Interaction of gossypol
with proteins from Spisula and bull sperms"
- Travel Award to International Congress on Chemical and Biological Aspects
of Vitamin B6 Catalysis, Turku, Finland (1987)
- Rockefeller Foundation Research Grant (1988) "Chemical studies on gossypol
with macromolecules from sperms, parasites, and viruses"
- Juvenile Diabetes Foundation International Research Grant (1989) "Nonenzymic
glycation of proteins"
- New York Heart Association Molly Berns Memorial Investigatorship Award
(1989)
- American Heart Association Research Grant (1990) "Chemical and genetic
engineering of hemoglobin functions"
- Rockefeller Foundation Research Grant (1990) "Gossypol action on Spisula
sperm"
- 旭硝子財団奨励賞 (1992)
- 三島海雲記念財団研究助成 (1992) 「初期メイラード反応における生化学的アプローチ」
- 日本性教育協会研究助成
(1992) 「精子運動阻害剤及び抗エイズ・ウイルス剤であるゴシポールの生化学的研究－基礎科学研究領域よりの性教育へのアピール」
- 日本生化学会JB論文賞 (1995) "Identification of active site lysyl residues of
phenylalanine dehydrogenase by chemical modification with methyl acetyl
phosphate combined with site-directed mutagenesis"
- 京都大学後援会助成 (1996)
- サントリー（株）研究助成 (2000) 「超臨界流体中での微生物反応」
- 浦上食品・食文化振興財団研究助成 (2000) 「香辛料は神経伝達物質合成酵素を刺激するか？」
- 中埜研究奨励会研究助成（2001）
「酵母のGABA合成酵素グルタミン酸デカルボキシラーゼの機能解析および高発現型酵母の探索」
- 旗影会研究助成（2001）「食品アレルギーとヒスタミン合成酵素の研究」
- うま味研究会研究助成（2005-2006）「味蕾におけるGABA合成酵素の味覚への関与を考える」
- （独）科学技術振興機構
地域イノベーション創出総合支援事業重点地域研究開発推進プログラム 平成18年度「シーズ発掘試験」
「天然物由来のヒスタミン合成酵素阻害剤の探索より新規抗ヒスタミン薬の開発研究」
- うま味研究会研究助成（2007-2008）「味蕾細胞内のタンパク質複合体形成の探索と味覚情報伝達に関与するタンパク質のマッピング」
- 日本味と匂学会 2007年度論文賞 "GABA is produced in taste bud"
- 日本味と匂学会 2008年度論文賞 「GABAの味覚テストによる味感覚への関与と香辛料成分によるGABA合成酵素の活性制御について」
- うま味研究会研究助成（2008-2009）「おいしさをそこなわず減塩に働きかける食品成分の探求について～GABA合成酵素の活性制御に関わる食品成分からのアプローチ～」（分担）
- 日本味と匂学会 2009年度論文賞 「味細胞の細胞型と応答特性」
- 日本農芸化学会 第7回農芸化学研究企画賞： （重点研究領域②「機能性食品素材および食品」）「減塩食品開発を目指して：天然物由来成分の塩味増強効果を定量的に評価するシステム開発」
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【原著論文】
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Purification of L-glutamate
decarboxylase from rat brain.Ann. Reports Grad. Sch. Human. & Sci., 23, 255-264 (2008)
|
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Effects of food components on the activity of GABA synthetic enzyme. Ann. Reports Grad. Sch. Human. & Sci., 23, 309-317 (2008)
|
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味蕾細胞におけるGABA合成－グルタミン酸の供給とGABAの利用－，ビタミン, 82, 387-394 (2008)
|
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Purification of L-glutamate
decarboxylase from monkey brain. Biosci. Biotech. Biochem., 72, 2269-2276 (2008)
|
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Inhibitory activity of Pimenta dioica extracts and constituents on recombinant
human histidine decarboxylase. Food Chem., 113, 445-449 (2009)
|
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加工済み赤身魚中に含まれるヒスタミンの検出法の検討，日本家政学会誌，59, 923-927 (2008)
|
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麹菌による茶葉の呈味改善，日本家政学会誌，59, 999-1004 (2008)
|
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ヒト由来ヒスチジンデカルボキシラーゼ活性を阻害する薬用植物のスクリーニング，日本家政学会誌，59,
1011-1016 (2008)
|
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味細胞の細胞型と応答特性，日本味と匂学会誌，15，285-288 (2008)
|
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グルタミン酸デカルボキシラーゼ（GAD）を発現する味蕾細胞におけるグルタミン酸取り込みの可能性とGABAの動向について，日本味と匂学会誌，15，325-328 (2008)
|
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マウスII型味細胞の発火頻度依存性ATP放出，日本味と匂学会誌，15，381-384 (2008)
|
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Expression of
glutamate decarboxylase isoform, GAD65, in human mononuclear leukocytes: A
possible implication of C-terminal end deletion by Western blot and RT-PCR
study. J. Biochem., 142, 633-638 (2007)
|
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Cells expressing GABA synthetic enzyme, glutamate decarboxylase, in stomach
and intestine: RT-PCR and immunohistochemistry studies. J. Biol. Macromol., 7, 55-62 (2007)
|
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Analysis of
intron-exon positioning on glutamate decarboxylase and its relation with
evolution. J.
Biol. Macromol., 7, 35-48 (2007)
|
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ヒスチジンデカルボキシラーゼ活性に及ぼす香辛料抽出物の阻害効果の検討，日本家政学会誌，58, 17-22 (2007).
|
|
Food components inhibiting recombinant human histidine decarboxylase activity.
J. Agr. Food Chem. 55, 299-304 (2007).
|
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GABA-synthesizing enzyme, GAD67, from dermal fibroblasts:
Evidence for a new skin function. Biochim. Biophys. Acta, 1770, 291-296 (2007).
|
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GABA is produced in taste bud. Chem. Senses, 32, J19 (2007), also 日本味と匂学会誌，13, 547-550 (2006)
|
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Bioinformatic study on histidine decarboxylase. J. Biol. Macromol., 6, 11-27
(2006).
|
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官能検査によるGABAの嗜好的側面の検討, 奈良女子大学家政学研究，53, 1-6 (2006).
|
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Resonance
assignments for the DNA binding domain of XPF-ERCC-1
heterodimer. J. Biomol. NMR, 32, 175 (2005).
|
|
Putative cis-elements in the promoter region of
the carrot phenylalanine ammonia-lyase gene induced during anthocyanin
synthesis. J.
Plant Res., 116, 155-159
(2003).
|
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Immunohistochemical study of the
localization of glutamate decarboxylase in rodent's submandibular gland. J.
Biol. Macromol., 2, 76-77 (2002).
|
|
Sub-zero temperature inactivation of
carboxypeptidase Y under high hydrostatic pressure. Eur. J. Biochem.,
269,
4666-4674 (2002).
|
|
Replacement of His12 or His119 of bovine
pancreatic ribonuclease A with acidic amino acid residues for the modification
of activity and stability. J. Biosci. Bioeng., 94, 39-44
(2002).
|
|
Amphipathic property of free sulfhydryl
group contributes to an increase in the catalytic efficiency of carboxypeptidase
Y. Eur.
J. Biochem., 269, 3220-3225 (2002).
|
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Increased proteolytic susceptibility of
carboxypeptidase Y caused by modification of the disulfide zipper. Biosci.
Biotechnol. Biochem., 66, 1393-1395
(2002).
|
|
Significance of the four carboxyl
terminal amino acid residues of bovine pancreatic ribonuclease A for structural
folding. J. Biochem., 131, 193-200 (2002).
|
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Gene cloning and characterization of
alanine racemases from Shigella dysenteriae, Shigella boydii,
Shigella flexneri, and Shigella sonnei. Biochem. Biophys. Res.
Commun., 288, 676-684 (2001).
|
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Substrate recognition mechanism of
carboxypeptidase Y. Biosci. Biotechnol. Biochem.,
65, 2465-2471 (2001).
|
|
Microanalysis
of GABA: An application for evaluating GABA
production in yeast strains and the effect of spice extracts on glutamate decarboxylase activity. J. Biol.
Macromol., 1, 45-48
(2001).
|
|
Structural and functional changes in bovine
pancreatic ribonuclease A by the replacement of Phe120 with other hydrophobic
residues. J. Biochem., 129, 917-922 (2001).
|
|
Interaction mode of H397A mutant
carboxypeptidase Y with protein substrates analyzed by the surface plasmon resonance.
Bull. Chem.
Soc. Japan, 73, 2587-2590
(2000).
|
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Expression of functional pancreatic
ribonuclease A in yeast: Comparative expression system between Saccharomyces cerevisiae and Pichia
pastoris. Biosci.
Biotechnol. Biochem.,
64, 2437-2444 (2000).
|
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Enzymatic and structural aspects on glutamate decarboxylase. J. Mol.
Catalysis B: Enzymatic, 10, 67-79 (2000).
|
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Effect of mutagenic replacement of carboxyl terminal
amino acid, Val124, on properties and regeneration of bovine pancreatic ribonuclease A.
J.
Biochem., 127, 877-881
(2000).
|
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Carboxypeptidase Y: Structural basis for protein sorting and catalytic triad.
J.
Biochem.,
126, 1-6
(1999).
|
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Contribution of the carbohydrate moiety to conformational
stability of the carboxypeptidase Y: High
pressure. Eur.
J. Biochem.,
262, 475-483
(1999).
|
|
Role
of carbohydrate moiety in carboxypeptidase Y:
Structural study of mutant enzyme lacking carbohydrate
moiety.
Biosci.
Biotechnol. Biochem., 63, 1045-1050
(1999).
|
|
Cathepsin A and
related enzymes in eukaryote（真核生物に共通のカテプシンA関連酵素），
生体の科学，50,
135-139 (1999).
|
|
The single
cysteinyl residue (Cys341) of carboxypeptidase Y for cavity control in the S1
substrate binding pocket. Advances in
High Pressure Bioscience & Biotechnology (Ludwig, H., ed), Springer,
Heidelberg, pp. 267-270 (1999).
|
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Use of high
pressure to investigate the role of carbohydrate moiety in the conformational
stability of carboxypeptidase Y. Advances in
High Pressure Bioscience & Biotechnology (Ludwig, H., ed), Springer,
Heidelberg, pp. 207-210 (1999).
|
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Expression of human histidine
decarboxylase in Saccharomyces
cerevisiae. J. Biochem.
Mol. Biol. Biophys., 2, 141-146
(1998).
|
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Proton-relay system of carboxypeptidase Y as a sole
catalytic site: Studies on mutagenic replacement of His397. J.
Biochem., 124, 446-450
(1998).
|
|
Role
of Phe120 on the activity and structure of bovine pancreatic ribonuclease
A. J.
Biochem., 124, 410-416
(1998).
|
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Bovine spleen cathepsin A:
Characterization and comparison with protective protein. J.
Biochem., 120, 701-706
(1998).
|
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High-pressure
effect on Cys341 substitution mutants of carboxypeptidase Y. High Pressure Biotechnology (S. Kunugi and
R. Hayashi, eds.), Sanei Shuppan, Kyoto, 29-36
(1998).
|
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Combined application of sub-zero temperature
and high pressure on biological materials. High
Pressure Food Science, Bioscience and Chemistry (N.S. Isaacs, ed.),
Royal Society of Chemistry, England, 166-174
(1998).
|
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Analysis of self-incompatibility-related ribonucleases
(S-RNases) in two species of pears, Pyrus communis and Pyrus
ussuriensis. Scientia
Horticulturae, 66, 159-167
(1996).
|
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A
novel heterologous gene expression system in Saccharomyces cerevisiae using the
isocitrate lyase promoter from Candida tropicalis. Appl. Microbiol. Biotechnol., 44, 759-765
(1996).
|
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A
large compressibility change of proteins induced by a
single amino acid substitution. Protein
Science, 5, 542-545 (1996).
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以下抜粋
|
Identification of the
catalytic histidine residue participating in the charge-relay system of
carboxypeptidase Y.
Protein
Science, 4, 2433-2435
(1995). |
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Identification of
active site lysyl residues of phenylalanine dehydrogenase by chemical
modification with methyl acetyl phosphate combined with site-directed
mutagenesis. J.
Biochem., 116, 1370-1376
(1994).
|
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Inactivation of
glutamate racemase of Pediococcus pentosaceus with L-serine
O-sulfate. Biosci.
Biotech. Biochem., 57, 1978-1979
(1993).
|
|
Random chemical
modification of the oxygen-linked chloride-binding sites of hemoglobin. Those in the central dyad axis may influence the transition between deoxy-
and oxy-hemoglobin. J.
Protein Chem.,
12, 561-570
(1993).
|
|
Kinetic and
stereochemical comparison of wild-type and active-site K145Q mutant enzyme of
bacterial D-amino
acid transaminase. J. Biol.
Chem., 268, 6932-6938
(1993).
|
|
Regulation of
serotonin-induced calcium uptake in Spisula oocytes tricyclic antidepressants. Neuroscience Lett., 151, 101-103
(1993).
|
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Effects of Methyl
acetyl phosphate, a covalent anti-sickling agent, on the density profiles of
sickle erythrocytes. J. Lab.
Clin. Med., 120, 152-158
(1992).
|
|
The functional,
oxygen-linked chloride binding sites of hemoglobin are contiguous within a channel in the central cavity. J. Protein Chem., 11, 177-185
(1992).
|
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Substitution of
glutamine for lysine at the pyridoxal phosphate binding site of bacterial D-amino
acid transaminase: Effects of exogenous
amines on the slow formation of intermediates.
J. Biol. Chem., 265, 22306-22312
(1990).
|
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Effects of D-serine
on bacterial D-amino
acid transaminase: Formation of a new
spectral band and inactivation of the enzyme.
Biochemistry, 28, 8798-8803
(1989).
|
|
Stereochemistry of
reactions catalyzed by bacterial D-amino
acid transaminase. J. Biol.
Chem., 264, 17784-17789
(1989).
|
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Methyl acetyl phosphate as a covalent probe for anion
binding sites in human and bovine hemoglobins.
J. Biol. Chem., 264, 12344-12351
(1989).
|
|
Sequence-dependent
reactivity of model peptides with glyceraldehyde. Carbohydr. Res., 189, 49-63
(1989).
|
|
g-Acetylenic-g-aminobutyrate
as an enzyme-activated inhibitor of D-amino
acid transaminase. Biochimie, 71, 505-508
(1989).
|
|
Inactivation of human immunodeficiency virus in vitro by gossypol. Contraception, 39, 579-587
(1989).
|
|
Some factors that
influence the non-enzymic glycation of peptides and
polypeptides by glyceraldehyde. J. Protein
Chem., 8, 299-315
(1989).
|
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Activity and spectroscopic properties of bacterial D-amino
acid transaminase after multiple site-directed mutagenesis of a single
tryptophan residue. Biochemistry, 28, 510-516
(1989).
|
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Site-directed mutagenesis of the cysteinyl residues and the active site
serine residue of bacterial D-amino
acid transaminase. Biochemistry, 28, 505-509
(1989).
|
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Properties
of carboxymethylated, cross-linked hemoglobin A. Biochemistry, 26, 5755-5761
(1987).
|
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Inhibition
of the gelation of extracellular and intracellular hemoglobin S by selective
acetylation with methyl acetyl phosphate.
Biochemistry, 26, 3125-3129
(1987).
|
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Pyrrolopyridine derivatives
from pyridoxal 5'-sulfate. Biochemistry, 21, 5212-5219
(1982).
|
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Chemistry of the
inactivation of cytosolic aspartate aminotransferase by serine-O-sulfate. Biochemistry, 21, 4387-4393
(1982).
|
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A novel
reaction of the coenzyme of glutamate decarboxylase with
L-serine-O-sulfate. Biochemistry, 21, 4377-4386 (1982).
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